Question

In deep sequencing, each of these raw data is unique. For 10 points each:
[10h] Give this term for the millions of base-called DNA sequences output by next-generation sequencers. Illumina and nanopore technologies respectively generate “short” and “long” types of these data.
ANSWER: sequencing reads [or read depth; or short-read or long-read sequencing]
[10e] Sanger sequencing, on the other hand, only gives one read per sample, which is why the monumental project to obtain this full sequence of every human chromosome took more than a decade.
ANSWER: the human genome [or the Human Genome Project or HGP]
[10m] Next-gen sequencing can read a genome in a day, and the slowest step is just waiting for one of these enzymes to attach adaptors. These enzymes form phosphodiester bonds and seal nicks at Okazaki fragments.
ANSWER: DNA ligases
<Adam Silverman, Science - Biology&gt; ~22897~ &lt;Editor: Adam Silverman>

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