Question
This technique is done using chemicals like Hoechst (“HOESHT”) 33342 to detect DNA by fluorescence microscopy. For 10 points each:
[10e] Name this common practice in histology. Crystal violet is used in a form of this technique invented by Hans Christian Gram to classify bacteria.
ANSWER: staining [or Gram staining; prompt on dying or dyes or coloring]
[10m] Hoechst dyes can stain both living cells and tissues that have undergone this process. This process kills cells, but preserves their organelles, and attaches them to a microscope slide.
ANSWER: fixation [or fixing]
[10h] Hoechst dyes attach to this structural feature of DNA. That’s odd, because transcription factors tend to avoid this side of the double helix, which is narrower than its complement in B-DNA.
ANSWER: minor groove [prompt on groove]
<David Bass, Science - Biology> ~22598~ <Editor: Adam Silverman>
Data
Summary
| Tournament | Edition | Exact Match? | Heard | PPB | Easy % | Medium % | Hard % |
|---|---|---|---|---|---|---|---|
| 2024 PACE NSC | 06/08/2024 | Y | 36 | 10.56 | 86% | 11% | 8% |